A cikin DNA na yau da kullun, RNA da haɗin haɗin acid nucleic waɗanda ba na halitta ba, matakin Karewa da haɗakarwa yana taka muhimmiyar rawa.
Mataki na Deprotection shine cire ƙungiyar DMT akan ingantaccen tallafi ko ƙungiyar 5' hydroxyl akan nucleoside da ta gabata tare da Organic acid, kuma an fallasa ƙungiyar hydroxyl don mataki na haɗin gwiwa mai zuwa.Acid trichloroacetic 3% a cikin dichloromethane ko toluene galibi ana amfani dashi don aiwatar da matakin kariya.Matsakaicin trichloroacetic acid da lokacin karewa (lokacin cirewa) yana mamaye tsabtar samfuran ƙarshe.Ƙananan maida hankali da rashin isasshen lokacin toshewa yana barin ƙungiyar DMT mara amsa, wanda ke rage yawan amfanin ƙasa kuma yana ƙara ƙazantattun da ba a so.Tsawon lokacin toshewa na iya haifar da depurine na jerin abubuwan da aka haɗa, haifar da ƙazantar da ba zato ba tsammani.
Matakin haɗakarwa yana kula da abun ciki na ruwa na kaushi da danshi a cikin iska.Matsakaicin ruwa a cikin kira ya kamata ya zama ƙasa da 40 ppm, mafi kyau ƙasa da 25 ppm.Don kiyaye yanayin haɗuwa da anhydrous, haɗin nucleic acid ya kamata a aiwatar da shi a cikin yanayin ƙarancin zafi, don haka muna ba da shawarar abokin cinikinmu don amfani daNarkar da Kayan Amidites, wanda zai iya narkar da foda ko mai mai Phosphoramite a cikin acetonitrile mai anhydrous don kauce wa haɗuwa da iska.
Tun da narkar da phosphoramidites yana da kyau a yanayin da ba ruwa ba, da tarko na kwayoyin don adsorb da ruwa a cikin reagents da amidite, yana buƙatar shiryaTarkon Kwayoyin Halitta.Muna ba da shawarar 2 g subsieve don 50-250ml reagent kwalabe, 5g don 250-500ml reagent kwalabe, 10g don 500-1000ml reagent kwalabe, da 20g ga 1000-2000ml reagent kwalabe.
Narkar da phosphoramidites ya kamata a gudanar a karkashin inert yanayi, da kuma maye gurbin activator reagents da acetonitrile ya kamata a gama a cikin lokaci.The Capping da Oxidation reagents ya kamata a yi amfani da wuri-wuri, da bude reagents ba da kasa shiryayye rai, da kuma kasa aiki a lokacin kira.
Lokacin aikawa: Agusta-09-2022