Dukansu DNA da RNA kira sun dogara ne akan dabarun haɗin lokaci mai ƙarfi da kuma phosphoramidite chemistry, ana iya amfani da mai yin DNA don haɗa RNA ko RNA analogues ba tare da ƙarin gyare-gyare ba, kuma ana iya amfani da reagents a cikin DNA kira kai tsaye a cikin RNA da wucin gadi nucleic acid. ' kira.Kamar yadda aka nuna a cikin Hoto 1, 2'-hydroxy a cikin RNA phosphoramite an kiyaye shi tare da ƙungiyar kariyar silyl, watau t-butyldimethylsilyl (TBDMS), yana hana halayen gefe akan ƙungiyar 2'-hydroxy mai rauni.Ƙungiya mai girma TBDMS ta hana amsawa tsakanin phosphoramidite da ƙungiyar 5'-hydroxy akan ingantaccen goyon baya, kuma ana buƙatar lokaci mai tsawo don tabbatar da gamsar da haɗin kai.
Hakazalika da kirar DNA, RNA da aka haɗa aka fara wargajewa daga ingantaccen tallafi ta hanyar aminolysis, sannan ƙungiyar TBDMS ta wargaje ta hanyar tetrabutylammonium fluoride (TBAF) ko trimethylamine trihydrofluoride.Ana iya tsarkake danyen RNA ta hanyar sake sakewa daga barasa da HPLC.
Hoto 1. Tsarin sinadarai na tubalan ginin asali a cikin DNA da haɗin RNA.
a) dABz phosphoramidite da b) rABz 2'-OTBDMS phosphoramidite.
Haɓaka siRNA magani yana buƙatar tsarin analogues na RNA na asali don haɓaka haɓakar halittu, 2'-hydroxy akan RNA na iya maye gurbin MeO, F da ƙungiyar MOE, kuma kulle nucleic acid (LNA) shima yana ba da kyakkyawan aiki a cikin jiyya na RNA. (Hoto na 2).Wadannan phosphoramidites suna ba da irin wannan aiki kamar nau'in DNA na phosphoramidites a cikin kira, kuma aikin aiki da tsarin tsarkakewa na waɗannan kwayoyin nucleic wadanda ba na halitta ba suna kama da DNA na asali.
Hoto 2. Tsarin sinadarai na tubalan gini a cikin magungunan siRNA.a) dABz 2-MeO phosphoramite;b) dABz 2-F phosphoramite;c) dABz 2-MOE phosphoramidite da d) dABz Kulle phosphoramidite.
Phosphoramiditessuna da mahimmanci don haɗa kwayoyin halitta, gami da galibin DNA da iyalan RNA da abubuwan da suka samo asali, duk phosphoramite da ke sama za mu iya bayarwa a cikin kunshin.
Muna ci gaba da haɓaka aikin samfuranmu, inganta tsarin samar da mu da samun cikakkun bayanai daidai.Ba wai kawai muna ba ku samfurori masu inganci ba, har ma muna ba ku horo da sabis.An sanye mu da ma'aikatan gudanarwa na ƙwararru da ƙwararrun ƙungiyar fasaha da ma'aikatan kulawa don samar wa abokan cinikinmu cikakkiyar sabis na tallace-tallace.
Lokacin aikawa: Agusta-09-2022