Ka'idar Oligo Synthesizer
A cikin fagagen nazarin halittun kwayoyin halitta da binciken kwayoyin halitta, ikon hada DNA yana taka muhimmiyar rawa.Haɗin DNA ya ƙunshi samar da ƙwayoyin DNA na wucin gadi ta hanyar tsara nucleotides a cikin takamaiman tsari.Don cimma wannan, masana kimiyya sun dogara da kayan aiki mai ƙarfi da aka sani da oligonucleotide synthesizer, wanda kuma aka sani da DNA synthesizer.
Oligonucleotide synthesizer wani nagartaccen kayan aiki ne wanda ke hada gajerun kwayoyin halittar DNA da ake kira oligonucleotides ta atomatik.Waɗannan gajerun igiyoyin DNA yawanci 10 zuwa 100 nucleotides ne a tsayi kuma sune mahimman tubalan gini a cikin aikace-aikace iri-iri da suka haɗa da sarrafa sarkar polymerase (PCR), haɗawar kwayoyin halitta, injiniyan kwayoyin halitta, da jerin DNA.
Oligonucleotide synthesizers aiki a kan ka'idar dabara da aka sani dam-lokaci kira.Dokta Marvin Caruthers mai lambar yabo ta Nobel ne ya fara yin wannan hanyar a cikin 1970s kuma an tace shi tsawon shekaru don haɓaka haɗin jerin DNA.Oligonucleotide kira ana aiwatar da shi ta mataki-mataki ƙari na ragowar nucleotide zuwa 5'-terminus na sarkar girma har sai an haɗa jerin da ake so.Kowace kari ana kiranta da zagayowar kira kuma ta ƙunshi halayen sinadarai guda huɗu:
Mataki na 1: De-tange (lalata) ------------Mataki 2: Haɗa-----Mataki na 3: Ci gaba ------------Mataki na 4: Oxidation
Ana maimaita wannan tsari ga kowane nucleotide har sai an sami jerin da ake so.Don tsayin oligonucleotides, wannan sake zagayowar na iya buƙatar maimaita sau da yawa don haɗa jerin duka. Ikon sarrafa daidaitaccen kowane mataki na sake zagayowar kira yana da mahimmanci ga mai haɗin oligonucleotide.Reagents da aka yi amfani da su, kamar nucleotides da masu kunnawa, suna buƙatar zama masu inganci don tabbatar da ingantaccen aiki mai inganci.Bugu da kari, synthesizers na buƙatar babban madaidaicin kula da zafin jiki da sauran yanayin muhalli don haɓaka halayen haɗin gwiwa da ake so da kuma hana halayen da ba a so.
Da zarar oligonucleotide ya gama haɗewa, yawanci ana katse shi daga ingantaccen tallafi kuma ana tsarkake shi don cire duk wasu ƙungiyoyin kariya ko ƙazanta.Zaɓuɓɓukan oligonucleotides ɗin suna shirye don aikace-aikacen ƙasa.
Ci gaban fasaha ya ba da damar haɓaka manyan na'urorin haɗin oligonucleotide masu iya haɗawa lokaci guda ɗaruruwa ko ma dubban oligonucleotides.Waɗannan kayan aikin suna amfani da fasahar haɗaɗɗun microarray, suna ba masu bincike damar samar da manyan ɗakunan karatu na oligonucleotide cikin sauri don dalilai na bincike iri-iri.
A taƙaice, ƙa'idodin da ke bayan masu haɗar oligonucleotide sun ta'allaka ne akan dabarun haɗa ƙarfi-lokaci, waɗanda suka haɗa da haɓaka matakin matakin nucleotides akan ingantaccen tallafi.Madaidaicin iko na sake zagayowar kira da reagents masu inganci suna da mahimmanci don ingantaccen aiki mai inganci.Masu haɗin Oligo suna taka muhimmiyar rawa a cikin bincike na DNA, yana ba wa masana kimiyya damar samar da oligonucleotides na al'ada don aikace-aikace iri-iri, yana ba da gudummawa ga ci gaba a cikin ilimin kimiyyar halittu da bincike na kwayoyin halitta.
Lokacin aikawa: Agusta-01-2023